hbmp2 capture biotin-conjugated detection antibodies  (R&D Systems)

Journal: Journal of controlled release : official journal of the Controlled Release Society

Article Title: Structural and functional characterization of a histidylated liposome for mRNA delivery.

doi: 10.1016/j.jconrel.2025.01.010

Figure Lengend Snippet: Fig. 5. Production of bioactive hBMP2 and hBMP9. (A) hBMP2 and (B) hBMP9 levels secreted by HEK293 and rMSC cells transfected with LFM or LYX lipoplexes containing cmRNA encoding hBMP-2 or hBMP-9. (C) Schematic representation of the BMP signaling activation pathway in C2C12-BRE/LUC cells, leading to firefly luciferase expression. (D) Quantification of the luciferase activity of C2C12-BRE/Luc cells treated with increasing doses of recombinant hBMP-2 or recombinant hBMP-9. (E) Quantification of the luciferase activity of C2C12-BRE/Luc cells transfected with increasing doses of LFM or LYX lipoplexes containing cmhBMP-2 or (F) cmhBMP-9 mRNA (n = 3). Values are represented as mean + SD. Two-way ANOVA statistical test was used (*) p < 0.05.

Article Snippet: The sequence of hBMP2 was cloned from pVAX1-BMP2, provided by G. Feichtinger (Addgene plasmid # 137909) [34].

Techniques: Transfection, Activation Assay, Luciferase, Expressing, Activity Assay, Recombinant

Journal: BMC Biotechnology

Article Title: A new mRNA structure prediction based approach to identifying improved signal peptides for bone morphogenetic protein 2

doi: 10.1186/s12896-024-00858-1

Figure Lengend Snippet: In vitro validation data (A) hBMP2 ELISA data from HEK293T cells. All computationally selected sequences showed non-significant increases in secretion. N = 7 ± SD, **** = p < 0.0001 (B) hBMP2 ELISA data from C2C12 cells. All computationally selected sequences showed decreased secretion versus the positive control, with several showing significant decreases. Particularly notable were the sequences that showed major decreases in the C2C12 results compared to the HEK293T experiment. These were SP1, SP2, SP hIL2 and SP hBMP2 TIS. N = 3 ± SD. * = p < 0.05, ** = p < 0.01, *** = p < 0.001 C) ALP assay data from C2C12 cells. All groups except the positive control showed background levels of ALP activity, indicating the novel SPs were not sufficient to induce osteogenesis in this context. N = 3 ± SD, *** = p < 0.001

Article Snippet: hBMP2 capture and biotin-conjugated detection antibodies, CHO-derived rhBMP2 standard and streptavidin-HRP working solution from the hBMP2 DuoSet ELISA system (DY355, R&D systems) were used for all experiments.

Techniques: In Vitro, Enzyme-linked Immunosorbent Assay, Positive Control, ALP Assay, Activity Assay

Journal: BMC Biotechnology

Article Title: A new mRNA structure prediction based approach to identifying improved signal peptides for bone morphogenetic protein 2

doi: 10.1186/s12896-024-00858-1

Figure Lengend Snippet: Overview of species, protein, sequence data and calculated RNA properties for the manually selected sequences

Article Snippet: hBMP2 capture and biotin-conjugated detection antibodies, CHO-derived rhBMP2 standard and streptavidin-HRP working solution from the hBMP2 DuoSet ELISA system (DY355, R&D systems) were used for all experiments.

Techniques: Sequencing

Journal: BMC Biotechnology

Article Title: A new mRNA structure prediction based approach to identifying improved signal peptides for bone morphogenetic protein 2

doi: 10.1186/s12896-024-00858-1

Figure Lengend Snippet: In vitro validation data (A) hBMP2 ELISA data from HEK293T cells. All computationally selected sequences showed non-significant increases in secretion. N = 7 ± SD, **** = p < 0.0001 (B) hBMP2 ELISA data from C2C12 cells. All computationally selected sequences showed decreased secretion versus the positive control, with several showing significant decreases. Particularly notable were the sequences that showed major decreases in the C2C12 results compared to the HEK293T experiment. These were SP1, SP2, SP hIL2 and SP hBMP2 TIS. N = 3 ± SD. * = p < 0.05, ** = p < 0.01, *** = p < 0.001 C) ALP assay data from C2C12 cells. All groups except the positive control showed background levels of ALP activity, indicating the novel SPs were not sufficient to induce osteogenesis in this context. N = 3 ± SD, *** = p < 0.001

Article Snippet: hBMP2 capture and biotin-conjugated detection antibodies, CHO-derived rhBMP2 standard and streptavidin-HRP working solution from the hBMP2 DuoSet ELISA system (DY355, R&D systems) were used for all experiments.

Techniques: In Vitro, Biomarker Discovery, Enzyme-linked Immunosorbent Assay, Positive Control, ALP Assay, Activity Assay

Journal: BMC Biotechnology

Article Title: A new mRNA structure prediction based approach to identifying improved signal peptides for bone morphogenetic protein 2

doi: 10.1186/s12896-024-00858-1

Figure Lengend Snippet: Overview of species, protein, sequence data and calculated RNA properties for the manually selected sequences

Article Snippet: hBMP2 capture and biotin-conjugated detection antibodies, CHO-derived rhBMP2 standard and streptavidin-HRP working solution from the hBMP2 DuoSet ELISA system (DY355, R&D systems) were used for all experiments.

Techniques: Sequencing

Journal: Frontiers in Cardiovascular Medicine

Article Title: BMP2 gene transfer induces pericardial effusion and inflammatory response in the ischemic porcine myocardium

doi: 10.3389/fcvm.2023.1279613

Figure Lengend Snippet: BMP2 transgene is successfully expressed in the pig myocardium after gene transfer. ( A ) Bottleneck stent in LAD caused ischemia and infarct of the anterior wall of the left ventricle and interventricular septum. Infarcted areas are marked with arrows. ( B ) Representative images of immunofluorescence staining of hBMP2 protein expression (BMP2 Ab, green) in the ischemic myocardium of AdLacZ- and AdBMP2-treated pigs at d6 after gene transfer (GT). Nuclei are stained with DAPI (blue). Scale bars, 100 µm. ( C ) Scoring of hBMP2 protein expression in AdLacZ- and AdBMP2-treated ischemic pig myocardium (five pigs/group, AdBMP2 = 16 samples, AdLacZ = 23 samples). hBMP2 protein was expressed in AdBMP2 (average score 2.53) but not in AdLacZ (average score 0.56)-treated hearts. Scores 0–3 were used, where 0 = absent, 1 = low, 2 = moderate, and 3 = high hBMP2 expression. The mean ± SD values are shown. The Mann–Whitney U test was used to determine the statistical significance. ** p < 0.01. ( D ) Representative H&E images of the ischemic myocardium of AdLacZ- and AdBMP2-treated pigs at d6 after GT. Images from the infract area (*), border of the GT area (GT edge), GT area, and left ventricle posterior wall (negative control of GT) and ischemia are shown. Scale bars, 100 µm.

Article Snippet: AdBMP2 transgene expression and secretion were confirmed using WB from the HUVEC media from samples collected at 24, 48, and 72 h post-transduction. hBMP2 antibody (LSBio, Seattle, WA, USA) was used to detect the transgene expression ( ).

Techniques: Immunofluorescence, Staining, Expressing, MANN-WHITNEY, Negative Control